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This auction is for a very clean Perkin Elmer GeneAmp PCR 9600 system. I plugged this unit in and as you can see it turned right on and everything seemed to fire up nicely. That is the extent of my testing capability however.
Model: 9600
Ser # N15776
Part # N801 - 0001
Refrig Type: MP-39 3.3oz Charge
30 psi low side, 155 psi high side.
General info from website:
Perkin Elmer GeneAmp 9600
Description: The GeneAmp PCR System 9600 automates the Polymerase Chain Reaction (PCR) technique for amplifying DNA. The Polymerase Chain Reaction (PCR) technique is conceptually a very simple method for amplifying nucleic acids. It mimics the natural DNA replication process in that the number of DNA molecules generated by the Polymerase Chain Reaction doubles after each cycle. In the PCR technique, the typical cycle consists of three steps: ? Denaturing the template DNA by heating it to a high temperature (94 degrees C to 95 degrees C), thereby producing two single strands of DNA. ? Annealing of the target-specific primers to the two separated DNA strands by cooling the reaction mixture to a lower temperature (37 degrees C to 65 degrees C). ? Extending the annealed primers with a DNA polymerase by warming the reaction mixture to an intermediate temperature (72 degrees C). The anneal and extend steps can be combined into a single setpoint, called two-temperature PCR. The original DNA segment can be either a small, discrete molecule whose sequence is already known or a part of a much larger molecule in a complex mixture, such as a chromosome fragment. The product of the reaction will be discrete double-stranded DNA molecules whose termini will be determined by the set of primers used. Because the copy number of the target DNA doubles after each cycle, amplification through 25 cycles would theoretically yield a 33 million fold increase of specific product. The PCR method readily lends itself to research applications. Cloning of the amplified sequence can be facilitated by extending the 5" end of the primers with short sequences harboring 1-3 restriction sites. Dideoxy sequencing can also be performed on the amplified product.
Features:
Reproducibility of cycle times Uniformity of PCR yields for samples Sample tray accommodating up to 96 samples in an 8 x 12 format Thin-walled MicroAmp Reaction Tubes allowing efficient heat transfer and improving equilibration times High speed of heating and cooling so that more samples can be run in a fixed amount of time, increasing productivity Heated cover assuring that tubes fit tightly in the wells Oil-free operation
These units are selling used online for over $2000.00 USD. I will palletize, band, shrink wrap and load for a handling fee of $25 USD. Item is fairly bulky and shipping via USPS is probably not the best method for this unit. I do have a 75% discount with RL Carriers and am happy to ship freight collect through them. Not responsible for any freight damage. Item sold as is. Local pick ups are encouraged and welcomed at no charge.
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