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Pinky and the Brain are cartoon characters who have starred in the American animated television series Animaniacs; Pinky and the Brain; and Pinky, Elmyra & the Brain.

Pinky and the Brain first appeared in 1993 as a recurring segment on the show Animaniacs. From 1995 to 1998, Pinky and the Brain were spun-off into their own Emmy-award-winning show on The WB Television Network, Steven Spielberg Presents Pinky and the Brain, with 65 episodes produced by Steven Spielberg and Warner Bros. Animation. Later, they appeared in the unsuccessful series, Steven Spielberg Presents Pinky, Elmyra & the Brain.

Pinky and Brain are genetically enhanced laboratory mice who reside in a cage in the Acme Labs research facility. Brain is self-centered and scheming; Pinky is good-natured but feebleminded. In each episode, Brain devises a new plan to take over the world, which ultimately ends in failure, usually due to Pinky's idiocy or the impossibility of Brain's plan. In common with many other Animaniacs shorts, many episodes are in some way a parody of something else, usually a film or novel. Nearly every episode opens with the following dialogue:

Pinky: "Gee, Brain, what do you want to do tonight?"
The Brain: "The same thing we do every night, Pinky—try to take over the world!"

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Steven Spielberg Presents Animaniacs, usually referred to as Animaniacs, is an American animated series, distributed by Warner Bros. and produced by Amblin Entertainment. The cartoon was the second animated series produced by the collaboration of Steven Spielberg and Warner Bros. Animation during the animation renaissance of the late 1980s and early 1990s. The studio's first series, Tiny Toon Adventures, was a success among younger viewers, and attracted a sizable number of adult viewers. A major precedent for the series was the success of Who Framed Roger Rabbit, which featured appearances by many of its famous cartoon characters, and was co-produced by Amblin Entertainment. The Animaniacs writers and animators, led by senior producer Tom Ruegger, used the experience gained from the previous series to create new animated characters that were cast in the mold of Chuck Jones and Tex Avery's creations.